Niche-dependent development of functional neuronal networks from embryonic stem cell-derived neural populations |
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Authors: | Sebastian Illes Stephan Theiss Hans-Peter Hartung Mario Siebler and Marcel Dihné |
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Institution: | (1) Department of Neurology, Heinrich-Heine University, Moorenstr. 5, 40225 D?sseldorf, Germany |
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Abstract: | Background The present work was performed to investigate the ability of two different embryonic stem (ES) cell-derived neural precursor
populations to generate functional neuronal networks in vitro. The first ES cell-derived neural precursor population was cultivated as free-floating neural aggregates which are known
to form a developmental niche comprising different types of neural cells, including neural precursor cells (NPCs), progenitor
cells and even further matured cells. This niche provides by itself a variety of different growth factors and extracellular
matrix proteins that influence the proliferation and differentiation of neural precursor and progenitor cells. The second
population was cultivated adherently in monolayer cultures to control most stringently the extracellular environment. This
population comprises highly homogeneous NPCs which are supposed to represent an attractive way to provide well-defined neuronal
progeny. However, the ability of these different ES cell-derived immature neural cell populations to generate functional neuronal
networks has not been assessed so far. |
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Keywords: | |
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