耐氧耐酸双歧杆菌的选育

采用驯化和紫外线诱变两种方法选育双歧杆菌耐氧耐酸菌株 .结果表明 ,通过诱变的方法选育耐氧耐酸菌株效果较为理想 .与原始菌株相比 ,将诱变菌株接种于pH 4.5的液体培养基中 ,在有氧或无氧条件下培养 ,个体形态均由短杆状变成球状 ,生长速度加快 ,在 36h时均达到最大活菌量 ,而且衰亡速度也明显减缓 ,在 72h时仍保持较高的活菌量 .

The breeding of oxygen - and acid - resistant bifidobacterium

Two methods, domestication and mutation induced by UV were used to breed the oxygen- and acid-resistant bifidobacterium. The results show that the mutative method is more ideal. Compared with original strains, when the mutants are cultured in pH 4.5 liquid medium, their morpholotype changes from short bacillus to coccus, their growth rate quickens and their living bacterial amount reaches the maximum at 36?h. Furthermore, their decrepit rate slows significantly and their living bacterial amount remains at the high level at 72?h.