An ICT-based approach to ratiometric fluorescence imaging of hydrogen peroxide produced in living cells |
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Authors: | Srikun Duangkhae Miller Evan W Domaille Dylan W Chang Christopher J |
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Institution: | Department of Chemistry, University of California, Berkeley, California 94720, USA. |
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Abstract: | We present the synthesis, properties, and biological applications of Peroxy Lucifer 1 (PL1), a new fluorescent probe for imaging hydrogen peroxide produced in living cells by a ratiometric response. PL1 utilizes a chemoselective boronate-based switch to detect hydrogen peroxide by modulation of internal charge transfer (ICT) within a 1,8-naphthalimide dye. PL1 features high selectivity for hydrogen peroxide over similar reactive oxygen species, including superoxide, and nitric oxide, and a 65 nm shift in emission from blue-colored fluorescence to green-colored fluorescence upon reaction with peroxide. Two-photon confocal microscopy experiments in live macrophages show that PL1 can ratiometrically visualize localized hydrogen peroxide bursts generated in living cells at immune response levels. |
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