Sample-pretreatment procedure for routine liquid chromatographic assay of serum cortisol |
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Authors: | Laganà A Rotatori M Vinci G Colombini M P Curini R |
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Institution: | Dipartimento di Chimica, Università degli Studi di Roma, Roma, Italy. |
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Abstract: | A specific method for measuring concentrations of cortisol in serum, by preliminary isolation on a "minicolumn" followed by elution and determination by liquid chromatography, is described. This assay requires only 500 microl of serum. The limit of determination of cortisol was found to be 5 microg l . The analytical recovery of cortisol added to serum ranged from 98 to 102%. The coefficients of variation ranged from 2.4 to 3.4% (within-day) and from 3.6 to 8.8% (day-to-day), depending on the cortisol concentration. The method compares well with a commonly used radioimmunological method. |
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