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Determination of the food carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in human hair by solid-phase extraction and gas chromatography-mass spectrometry |
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| Authors: | S Hegstad E Lundanes R Reistad L S Haug G Becher J Alexander |
| Institution: | 1. Department of Environmental Medicine, National Institute of Public Health, Torshov, PO Box 4404, 0403, Oslo, Norway 2. Department of Chemistry, University of Oslo, Blindern, PO Box 1033, 0315, Oslo, Norway 3. National Institute for Consumer Research, PO Box 173, 1325, Lysaker, Norway 4. Norwegian Institute of Water Research, Kjels?s, PO Box 173, 0411, Oslo, Norway |
| Abstract: | Summary A method for the determination of the food mutagen 2-amino-1-methyl-6-phenylimidazo4,5-b]pyridine (PhIP) in human hair has been developed and validated. Hair samples (200 mg) were dissolved in NaOH (1 M) and PhIP was isolated by successive solid-phase extraction on a polystyrene-divinylbenzene column and on a silica-based mixed-mode column with C8 and-SO3 − functional groups. Quantification was performed by gas chromatography-electron-impact ionization high-resolution mass spectrometry in selected-ion-monitoring mode. The method was validated for determination of PhIP in the concentration range 0.5–25 ng g−1 hair with 2H3]PhIP as internal standard. The limit of quantification was 0.26 ng g−1 hair. Within-day and between-day precision were in the ranges 1–27% and 2–15% relative standard deviation, respectively. The hair sample used for method validation was found to contain 0.26 ng PhIP g−1 hair. |
| Keywords: | Gas chromatography-mass spectrometry Solid-phase extraction Human hair 2-Amino-1-methyl-6-phenylimidazo[4 5-b]pyridine (PhIP) |
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