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| 响应面分析法优化苏云金芽孢杆菌 BRC-ZQL3菌株发酵培养基 | |
| 引用本文： | 张群林,张志国,余洁,林群新,吴小凤,关雄.响应面分析法优化苏云金芽孢杆菌 BRC-ZQL3菌株发酵培养基[J].福建师范大学学报(自然科学版),2011,27(5):85-90. |
| 作者姓名： | 张群林 张志国 余洁 林群新 吴小凤 关雄 |
| 作者单位： | 1. 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002;福州第一中学,福建福州350001 2. 福州第一中学,福建福州,350001 3. 福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002;福建农林大学生命科学学院,福建福州350002 |
| 基金项目： | 科技部农业科技成果转化基金(2019GB2C400212); 福建省高校服务海西建设重点项目(闽教高(2009)5号); 福建省青年人才项目(2008F30201) |
| 摘 要： | 从地衣分离获得了Bt菌株BRC-ZQL3,为提高其产孢水平,应用数学统计方法对该菌株发酵培养基进行优化.通过单因子分析,研究碳源、氮源、初始pH等因子对产孢的影响;再采用2水平PlackettBurman设计,筛选影响产孢的8个因子,实验结果表明培养基成分中3个重要影响的因子,分别为CaCO3、玉米粉、酵母膏;利用响应... |
| 关 键 词： | 苏云金芽孢杆菌 Plackett-Burman设计 响应面分析法 |
Use of Response Surfacesnalysis Methodology to Optimize Fermentation Culture Medium for Bacillus thuringiensis Strain BRC-ZQL3 |
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| ZHANG Qun-lin,ZHANG Zhi-guo,YU Jie,LIN Qun-xin,WU Xiao-feng,GUAN Xiong.Use of Response Surfacesnalysis Methodology to Optimize Fermentation Culture Medium for Bacillus thuringiensis Strain BRC-ZQL3[J].Journal of Fujian Teachers University(Natural Science),2011,27(5):85-90. | |
| Authors: | ZHANG Qun-lin ZHANG Zhi-guo YU Jie LIN Qun-xin WU Xiao-feng GUAN Xiong |
| Institution: | ZHANG Qun-lin1,2,ZHANG Zhi-guo2,YU Jie1,LIN Qun-xin1,3,WU Xiao-feng1,GUAN Xiong1,3(1.Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agricultureand Forestry University,Fuzhou 350002,China,2.Fuzhou No.1 Middle School,Fuzhou 350001,3.College of Life Sciences,Fujian Agriculture and Forestry University,China) |
| Abstract: | In order to improve the spore yield of the Bt strain BRC-ZQL3 isolated from Lichens,the mathematical-statistics method was employed to optimize the fermentation medium for spore production of BRC-ZQL3.The mono-factor experiment method was adopted to determine the effects of different carbon and nitrogen sources,initial pH and other factors on sporulation of BRC-ZQL3.The three important factors CaCO3,corn meal and yeast extract were found after the screening of eight factors by applying the Plackett-Burman e... |
| Keywords: | Bacillus thuringiensis Plackett-Burman response surface snalysis |
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