In vitro photodynamic antimicrobial chemotherapy in dentine contaminated by cariogenic bacteria |
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Authors: | M A S Melo D M de-Paula J P M Lima F M C Borges C Steiner-Oliveira M Nobre-dos-Santos I C J Zanin E B Barros and L K A Rodrigues |
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Institution: | 1.Faculty of Pharmacy, Dentistry and Nursing,Federal University of Ceará,Fortaleza, CE,Brazil;2.Piracicaba Dental School,University of Campinas,Piracicaba, SP,Brazil;3.Faculty of Dentistry,Federal University of Ceará,Sobral, CE,Brazil;4.Department of Physics,Federal University of Ceará,Fortaleza, CE,Brazil |
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Abstract: | The development of a method to ensure bacterial-free substrates without extensive cavity preparation would be highly useful
to dentistry, since there is no currently available effective method for killing residual bacteria in dentinal tissue. This
randomized in vitro study determined parameters for using toluidine blue O (TBO) with a light-emitting diode (LED) for dentine
caries disinfection and monitored intrapulpal/periodontal temperatures during irradiation. Occlusal human dentine slabs were
immersed in Streptococcus mutans culture for demineralization induction. Slabs were allocated to 10 groups (n = 15), which were treated with 0.1 mg ml−1 TBO with 5 min of incubation time or 0.9% NaCl solution for 5, 10 or 15 min, and submitted or not to irradiation for 5, 10
or 15 min (47, 94, and 144 J/cm2). Before and after treatments, dentine samples were analyzed with regard to S. mutans counts. In whole teeth, temperature in pulp and periodontium was measured by thermocouples during irradiation. Kruskal-Wallis/Student-Newman-Keuls,
and ANOVA/Tukey test were respectively utilized to compare log reductions and temperature rises between groups. Bacterial
reduction was observed when dentine was exposed to both TBO and LED at all irradiation times, as well as to LED alone for
10 and 15 min. Temperature increases lower than 2°C were observed for either pulp or periodontium. Concluding, LED combined
with TBO is a safe and effective approach for dentine caries disinfection. Nevertheless, additional studies should be conducted
to determine the influence of the irradiation in S. mutans viability in dentinal surface/tubules. |
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