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Enzymatic synthesis of iso-globotriose from partially protected lactose |
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| Authors: | Lenka Weignerov Petr Sedmera Zdenka Hu&#x;kov Petr Halada Vladimír K&#x;en Monica Casali Sergio Riva |
| Institution: | aInstitute of Microbiology, Academy of Sciences of the Czech Republic, Laboratory of Biotransformation, Vídenská 1083, CZ 142 20 Prague 4, Czech Republic;bIstituto di Biocatalisi e Riconoscimento Molecolare, CNR, Via Mario Bianco 9, I 20131 Milano, Italy |
| Abstract: | 6′-O-Acyl-lactose derivatives (1b-d) were prepared by selective enzymatic acylation of lactose using the protease subtilisin. These compounds were used as acceptors for enzymatic transglycosylations catalysed by an α-galactosidase from Talaromyces flavus forming (after deacylation) iso-globotriose (α-d-Galp-(1→3)-β-d-Galp-(1→4)Glc, 2a). |
| Keywords: | enzymatic acylation enzymatic transglycosidation regioselectivity α -galactosidase |
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