| Molar Mass Distribution and Chain Conformation of Polysaccharides from Fortunella margarita （Lour.） Swingle |
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| 引用本文： | 曾红亮,张怡,刘骏,郑宝东. Molar Mass Distribution and Chain Conformation of Polysaccharides from Fortunella margarita （Lour.） Swingle[J]. 结构化学, 2014, 33(8): 1245-1252 |
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| 作者姓名： | 曾红亮 张怡 刘骏 郑宝东 |
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| 作者单位： | College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| 基金项目： | Supported by Science and Technology Plan Major Project of Fujian Province(2013Y0003);Agricultural Products(fruits and vegetables) Processing Engineering Technology Research Center Funding Projects of Fujian Province(2009N2002);Scientific and Technological Innovation Team Support Plan of Institution of Higher Learning in Fujian Province([2012]03);Scientific and Technological Innovation Team Support Plan of Fujian Agriculture and Forestry University(cxtd12009) |
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| 摘 要： | In order to study the effects of different extraction methods on the molar mass distribution and chain conformation of Fortunella margarita(Lour.) Swingle polysaccharides(FP), we used extraction by hot water(WFP), ultrasonic-assisted treatment(UFP), microwave-assisted treatment(MFP) and ultrasonic/microwave-assisted treatment(UMFP), and then Fourier transform infrared(FT-IR) spectroscopy as well as 1H and 13 C nuclear magnetic resonance(NMR) spectroscopy to characterize the structural properties of FP extracted. The molar weight(Mw), polydispersity index(Mw/Mn), root-mean-square(RMS) turning radius(Rg), molar mass distribution and chain conformation of FP were studied systematically using size-exclusion chromatography(SEC), multi-angle laser light-scattering(MALLS) and refractive index(RI). WFP, UFP, MFP and UMFP are all typical carbohydrates according to 1H NMR, 13 C NMR and FT-IR measurements. The type of glycosidic linkage is mainly a β-glycosidic bond with a small amount of α-glycosidic bond. The results obtained by the SEC-MALLS-RI system showed the molar masses of WFP and UMFP were distributed mainly in the range of 5.0×106~1.0×107 g·mol–1 and they accounted for 57.80% and 56.84% of total FP, respectively. The molar masses of UFP and MFP were distributed mainly in the 1.0×106~5.0×106 g·mol–1 range, which accounted for 38.24% and 52.39% of FP, respectively. WFP and UMFP in water were uniform spherical polymers; UFP and MFP were typical highly branched polymers and the degree of branching for MFP was higher compared to UFP. These results indicated the ultrasonic- and microwave-assisted extraction methods caused a significant decrease of the molar mass of FP but the ultrasonic/microwave synergistic extraction method had no effect.
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| 关 键 词： | 链构象 分子量分布 金桔 多糖 摩尔质量分布 傅立叶变换红外光谱 提取方法 微波辅助 |
Molar Mass Distribution and Chain Conformation of Polysaccharides from Fortunella margarita(Lour.) Swingle |
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| ZENG Hong-Liang , ZHANG Yi , LIU Jun , ZHENG Bao-Dong. Molar Mass Distribution and Chain Conformation of Polysaccharides from Fortunella margarita(Lour.) Swingle[J]. Chinese Journal of Structural Chemistry, 2014, 33(8): 1245-1252 |
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| Authors: | ZENG Hong-Liang ZHANG Yi LIU Jun ZHENG Bao-Dong |
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| Affiliation: | College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China |
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| Abstract: | Fortunella margarita （Lour.） Swingle, polysaccharides,molar mass distribution, chain conformation |
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| Keywords: | Fortunella margarita(Lour.) Swingle polysaccharides molar mass distribution chain conformation |
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