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Nanoparticle‐Mediated Binning and Profiling of Heterogeneous Circulating Tumor Cell Subpopulations |
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| Authors: | Dr Reza M Mohamadi Justin D Besant Adam Mepham Brenda Green Dr Laili Mahmoudian Thaddeus Gibbs Dr Ivaylo Ivanov Anahita Malvea Jessica Stojcic Alison L Allan Lori E Lowes Prof Edward H Sargent Robert K Nam Prof Shana O Kelley |
| Institution: | 1. Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON (Canada);2. Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON (Canada);3. Sunnybrook Research Institute, Toronto, ON (Canada);4. London Health Sciences Centre, London, ON (Canada);5. Department of Electrical and Computer Engineering, University of Toronto, Toronto, ON (Canada);6. Department of Biochemistry, University of Toronto, Toronto, ON (Canada) |
| Abstract: | The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters blood‐borne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial‐to‐mesenchymal transition (EMT). |
| Keywords: | cancer cell analysis cell sorting magnetic nanoparticles microfluidics tumor cells |