Chirality‐Assisted Ring‐Like Aggregation of Aβ(1–40) at Liquid–Solid Interfaces: A Stereoselective Two‐Step Assembly Process |
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| Authors: | Guanbin Gao Prof Mingxi Zhang Dr Pei Lu Dr Guanlun Guo Prof Dong Wang Prof Taolei Sun |
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| Institution: | 1. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070 (PR China);2. Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190 (PR China);3. Hubei Key Laboratory of Advanced Technology of Automotive Components, Wuhan University of Technology, Wuhan 430070 (PR China);4. School of Chemistry, Chemical Engineering and Life Science, Wuhan University of Technology, Wuhan 430070 (PR China) |
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| Abstract: | Molecular chirality is introduced at liquid–solid interfaces. A ring‐like aggregation of amyloid Aβ(1–40) on N‐isobutyryl‐L ‐cysteine (L ‐NIBC)‐modified gold substrate occurs at low Aβ(1–40) concentration, while D ‐NIBC modification only results in rod‐like aggregation. Utilizing atomic force microscope controlled tip‐enhanced Raman scattering, we directly observe the secondary structure information for Aβ(1–40) assembly in situ at the nanoscale. D ‐ or L ‐NIBC on the surface can guide parallel or nonparallel alignment of β‐hairpins through a two‐step process based on electrostatic‐interaction‐enhanced adsorption and subsequent stereoselective recognition. Possible electrostatic interaction sites (R5 and K16) and a chiral recognition site (H14) of Aβ(1–40) are proposed, which may provide insight into the understanding of this effect. |
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| Keywords: | amyloids chirality stereoselectivity surface assembly surface interactions |
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