首页 | 本学科首页   官方微博 | 高级检索  
     


Development and application of fluorescence polarization assays in drug discovery
Authors:Burke T J  Loniello K R  Beebe J A  Ervin K M
Affiliation:PanVera LLC, 501 Charmany Drive, Madison, WI 53719, USA. tcburke@tds.net
Abstract:Fluorescence polarization technology has been used in basic research and commercial diagnostic assays for many decades, but has begun to be widely used in drug discovery only in the past six years. Originally, FP assays for drug discovery were developed for single-tube analytical instruments, but the technology was rapidly converted to high-throughput screening assays when commercial plate readers with equivalent sensitivity became available. This review will discuss fluorescence polarization assays in current use in drug discovery research as well as those in development that will likely be used in the near future. These assays include targets such as kinases, phosphatases, proteases, G-protein coupled receptors, and nuclear receptors.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号