基于甲酸和氢氧化钠的衍生移动反应界面的数值计算与实验验证(英文)

在甲酸（α相）和氢氧化钠（γ相）缓冲液形成的移动反应界面的基础上，提出了一种衍生移动反应界面模型。模型表明在α相和γ相之间会形成一个新的β相，β相和α相形成衍生移动反应界面，β相和γ相形成移动界面。为了验证该模型的有效性，该文给出了相关理论及数值推导过程。此外，基于毛细管电泳和自制装置进行了相关实验。结果表明，若使用以前的移动反应界面，实验结果与理论计算存在较大误差，而采用该文提出的衍生移动反应界面，实验数据与理论计算结果高度一致。该文提出的衍生移动反应界面理论及模型对于电泳，特别是毛细管电泳中样品的分离与富集具有重要的意义。

Numerical computation and experimental verification of a derivatized moving reaction boundary originally created with formic buffer and sodium hydroxide

A model of derivatized moving reaction boundary (MRB) is formulated from original MRB formed with formic buffer (phase α) and sodium hydroxide (phase γ). The model shows the formation of a new phase (phase β) with high pH value originated from phase γ, derivatized MRB created with phases α and β, as well as stationary boundary formed between phases β and γ. To demonstrate the validity of this model, the theoretical and numerical procedures are advanced. At the same time, the experimental procedures are also developed relied on a capillary electrophoresis (CE) and a home-made apparatus. There are evident systemic errors and low agreements between the experimental results and theoretical calculation with the original model of MRB developed in our previous papers. However, there are much high agreements between the experiments and theoretical computation relied on the model of derivatized MRB developed herein. The model of derivatized MRB together with the relative theoretical and experimental procedures holds evident significance for the design of the new separation and compressive techniques of samples used widely in electrophoresis including CE.