| 开管毛细管色谱柱的制备及其在蛋白质组学研究中的应用进展 |
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| 引用本文： | 周珊珊,翟睿,焦丰龙,郝斐然,吕雅瑶,钱小红,张养军.开管毛细管色谱柱的制备及其在蛋白质组学研究中的应用进展[J].色谱,2016,34(3):226-231. |
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| 作者姓名： | 周珊珊 翟睿 焦丰龙 郝斐然 吕雅瑶 钱小红 张养军 |
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| 作者单位： | 1. 广西医科大学, 广西 南宁 530021;
2. 军事医学科学院放射与辐射医学研究所, 北京蛋白质组研究中心, 蛋白质组学国家重点实验室, 北京 102206 |
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| 基金项目： | 国家重大科学计划项目(2012CB910603);国家高技术研究发展规划项目(2012AA020202);国家重大科学仪器设备开发专项项目(2012YQ12004407,2011YQ06008408,2013YQ14040506);国家自然科学基金项目(21275519,20735005,31100591). |
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| 摘 要： | 近年来随着生命科学等领域的深入发展,人们对微量样本分离分析的需求越来越高,液相色谱系统的微量化受到了更多的关注。由于开管毛细管色谱柱具有较低的反向压力,可通过采用更长的色谱柱提高柱效,从而实现对复杂生物样本的高效分离,因而成为液相色谱柱新的发展方向。本文对开管毛细管色谱柱的制备方法及应用进展进行了综述。
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| 关 键 词： | 蛋白质组学 多孔层 开管毛细管色谱柱 毛细管液相色谱 综述 |
| 收稿时间： | 2015-11-18 |
Progress in preparation of open tubular columns in capillary liquid chromatography and their applications in proteomics studies |
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| ZHOU Shanshan,ZHAI Rui,JIAO Fenglong,HAO Feiran,LV Yayao,QIAN Xiaohong,ZHANG Yangjun.Progress in preparation of open tubular columns in capillary liquid chromatography and their applications in proteomics studies[J].Chinese Journal of Chromatography,2016,34(3):226-231. |
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| Authors: | ZHOU Shanshan ZHAI Rui JIAO Fenglong HAO Feiran LV Yayao QIAN Xiaohong ZHANG Yangjun |
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| Institution: | 1. Guangxi Medical University, Nanning 530021, China;
2. State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China |
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| Abstract: | With the further development of life sciences in recent years, the demand for analysis of very small amounts of sample has increased, and more attention has been paid for the miniaturized liquid chromatography system. Longer open tubular capillary columns were selected to increase column efficiency due to their low column back pressure, and they were often used to separate complex biological samples in high resolution. Therefore, they emerged as the new area of liquid chromatography columns. The preparation methods and applications in proteomics of open tubular columns in capillary liquid chromatography (capLC) are reviewed. |
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| Keywords: | capillary liquid chromatography (capLC) open tubular capillary columns porous layer proteomics review |
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