聚胍基离子液体材料制备及其对蛋白质识别性能评价

磷酸化修饰是蛋白质翻译后修饰中最为重要的修饰之一,蛋白质的磷酸化修饰几乎参与生命活动的每一个环节。因此,制备对磷酸化蛋白具有选择性识别性能的材料在磷酸化蛋白质组学中具有重要意义。本实验首先合成胍基离子液体功能单体,通过沉淀聚合法合成聚胍基离子液体材料。通过傅里叶红外光谱(FT-IR)、扫描电子显微镜(SEM)、热重分析仪(TGA)考察了材料的结构、形貌、热稳定性。结果显示所制备材料为粒径约200 nm的球形颗粒。并以标准磷酸化蛋白(β-酪蛋白)为模型蛋白质,考察了聚胍基离子液体材料的识别性能。研究结果表明:材料对磷酸化蛋白具有较高吸附容量(对 β-酪蛋白的最大吸附量达到599.1 mg/g)、较快的吸附速度(1 h内达平衡),而且对磷酸化蛋白表现出较高的选择性。

Preparation of poly(guanidinium ionic liquid)s materials and evaluation of their recognition properties for protein

Protein phosphorylation is one of the most important post-translational modifications in life activities, which has a close relationship to the precise regulation of life activities. Thus, preparation of materials capable of selective enrichment of phosphoproteins is significantly important to phosphoproteome research. In this research, we prepared poly(guanidinium ionic liquid)s materials by using a synthesized guanidinium ionic liquid functional monomer. Poly(guanidinium ionic liquid)s materials were characterized by Fourier transform infrared spectrometer (FT-IR), scanning electron microscope (SEM) and thermo gravimetric analyzer (TGA). The results indicated that microspheres had a mean size of 200 nm in diameter. Using β-casein as a model phosphoprotein, the recognition properties of the obtained materials for phosphoprotein were evaluated. The adsorption results showed that microspheres not only had a high adsorption capacity for phosphorylation proteins (599.1 mg/g for β-casein) and a rapid balance in 1 h, but also displayed high selectivity to phosphorylation proteins.