Solid-supported synthesis,deprotection and enzymatic purification of oligodeoxyribonucleotides |
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| Affiliation: | 1. Institute for Biodiversity Science and Sustainability, California Academy of Sciences, San Francisco, CA 94118, United States;2. Department of Biology, San Francisco State University, San Francisco, CA 94132, United States;3. University of Alaska Museum, Fairbanks, AK 99775, United States;4. Pest Management Centre, African Centre of Excellence, Sokoine University of Agriculture, Morogoro, Tanzania;5. Science & Education, Field Museum of Natural History, Chicago, IL 60605, United States;1. Faculty of Chemistry, Adam Mickiewicz University, Umultowska St 89 b, 61-614 Poznań, Poland;2. Department of Pharmacology, Poznań University of Medical Sciences, Rokietnicka St 5 a, 60-806 Poznań, Poland |
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| Abstract: | A simple solid-phase scheme for deprotection and enzymatic purification of oligodeoxyribonucleotides synthesized by a phosphoramidite method using hydrazine-sensitive protection is presented. |
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