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Implementation of a Gaussian Beam Laser and Aspheric Optics for High Spatial Resolution MALDI Imaging MS |
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| Authors: | Andre Zavalin Junhai Yang Andreas Haase Armin Holle Richard Caprioli |
| Institution: | 1. Mass Spectrometry Research Center, National Research Resource for Imaging Mass Spectrometry, Vanderbilt University, Nashville, TN, USA 2. Department of Biochemistry, Vanderbilt University, Nashville, TN, USA 3. Bruker Daltonik GmbH, Bremen, Germany 4. Departments of Pharmacology, Medicine, and Chemistry, Vanderbilt University, Nashville, TN, USA |
| Abstract: | We have investigated the use of a Gaussian beam laser for MALDI Imaging Mass Spectrometry to provide a precisely defined laser spot of 5 μm diameter on target using a commercial MALDI TOF instrument originally designed to produce a 20 μm diameter laser beam spot at its smallest setting. A Gaussian beam laser was installed in the instrument in combination with an aspheric focusing lens. This ion source produced sharp ion images at 5 μm spatial resolution with signals of high intensity as shown for images from thin tissue sections of mouse brain. ? |
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