Investigation of the Interaction Between Ofloxacin and Bovine Serum Albumin: Spectroscopic Approach |
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Authors: | Yan-Jun Hu Yu Ou-Yang Ai-Min Bai Wei Li Yi Liu |
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Affiliation: | (1) College of Chemistry, Liaoning University, Shenyang, 110036, People’s Republic of China;(2) School of Pharmaceutical Science, Liaoning University, Shenyang, 110036, People’s Republic of China |
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Abstract: | Ofloxacin is an antibacterial compound that belongs to the fluoroquinolone family. In this paper, the interaction between ofloxacin and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and UV-Vis absorbtion spectroscopy under approximately the human physiological conditions. The thermodynamic parameters were calculated according to the dependence of enthalpy change on the temperature as follows: ΔH has a small negative value (−9.96 kJ⋅mol−1), whereas ΔS has a positive value (54.77 J⋅mol−1⋅K−1). In this work, it was proved that the fluorescence quenching of BSA by ofloxacin is a result of the formation of an ofloxacin–BSA complex. Binding studies concerning the number of binding sites (n=1.14) and apparent binding constant were performed by Scatchard’s procedure. The binding distance r between donor (BSA) and acceptor (ofloxacin) was obtained according to the fluorescence resonance energy transfer (FRET) method. |
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