Structure and mobility of lipid membranes on a thermoplastic substrate |
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Authors: | Hochrein Marion B Reich Christian Krause Bärbel Rädler Joachim O Nickel Bert |
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Affiliation: | Department für Physik, Ludwig-Maximilians-Universit?t München, Geschwister-Scholl-Platz 1, 80539 München, Germany. marion.hochrein@physik.uni-muenchen.de |
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Abstract: | Supported lipid membranes constitute one of the most important model systems for cell membranes. The properties of lipid membranes supported by the hydrophobic solid polymer cyclic olefin copolymer (COC) were investigated. Lipid layers consisting of varying amounts of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP, cationic) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, neutral) prepared by vesicle fusion and solvent exchange were compared. All lipid mixtures coated the COC surface homogeneously forming a fluid membrane as verified by fluorescence microscopy and fluorescence recovery after photobleaching (FRAP). The exact structure of the supported membranes was determined by synchrotron reflectivity experiments using a microfluidic chamber. The X-ray data are in agreement with a compressed (head-to-head distance = 29 angstroms) and less densely packed bilayer. |
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