Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection |
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Authors: | H. B. Li F. Chen |
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Affiliation: | (1) Department of Botany, The University of Hong Kong, Pokfulam Road, Hong Kong |
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Abstract: | Summary A novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol-KH2PO4 buffer (0.1 M, pH 7.0)-water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL−1 to 0.5 μg mL−1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%. |
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Keywords: | Column liquid chromatography Pharmaceutical preparations Water- and fat-soluble vitamins |
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