Effect of poly(hydroxybutyrate-co-hydroxyhexanoate) microparticles on growth of murine fibroblast L929 cells |
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Authors: | Shan Cheng Yan Zhao Guo-Qiang Chen |
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Affiliation: | a Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China b Multidisciplinary Research Center, Shantou University, Shantou, Guangdong 515063, China |
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Abstract: | Poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) microparticles were successfully prepared and their proliferative effects on cultured fibroblasts were studied. PHBHHx microparticles (0.005-0.1 g/L) promoted cell proliferation in murine fibroblast L929 and elevated intracellular calcium concentrations ([Ca2+]i). EGTA (ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid) inhibited PHBHHx microparticle-induced cell proliferation by chelating the extracellular Ca2+ and blocking the PHBHHx particle-induced [Ca2+]i increase. Transwell experiments demonstrated that PHBHHx microparticles stimulated fibroblast proliferation when separated from cells by a 0.4 μm filter as effectively as when applied directly to cells. Since PHBHHx microparticles had a diameter of 75 μm, the stimulatory effect of PHBHHx particles on cell growth was attributed to degradation products smaller than 0.4 μm in diameter. The trophic effect of these microparticles is consistent with our previous reports demonstrating good biocompatibility for PHBHHx. |
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Keywords: | PHB PHBHHx microparticles Fibroblast L929 Proliferation Biodegradation |
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