| Amplified Immunoassay of Human IgG Using Real—time Biomolecular Interaction ANalysis （BIA） Technology |
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| 引用本文： | 裴仁军 崔小强 等. Amplified Immunoassay of Human IgG Using Real—time Biomolecular Interaction ANalysis （BIA） Technology[J]. 中国化学, 2002, 20(5): 441-446. DOI: 10.1002/cjoc.20020200507 |
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| 作者姓名： | 裴仁军 崔小强 等 |
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| 作者单位： | [1]LaboratoryofElectroanalyticalChemistryandNationalAnalyticalResearchCenterofElectrochemistryandSpectroscopy,ChangchunInstituteofAppliedChemistry,ChineseAcademyofSciences,Changchun,Ji [2]LaboratoryofElectr,ChangchunInstituteofAppliedChemistry,ChineseAcademyofSciences,Changchun,Ji |
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| 基金项目： | ProjectsupportedbytheNationalNaturalScienceFoundationofChina (Nos.2 0 0 75 0 2 7and 2 9835 12 0 ) |
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| 摘 要： | An automated biomolecular interaction analysis instrument(BIAcore)based on surface plasmon resonance(SPR)has been used to determine human immunoglobulin G(IgG) in real time.Polyclonal anti-human IgG antibody was covalently immobilized to a carboxymethyldextran-modified gold film surface.The samples of human IgG prepared in HBS buffer were poured over the immpbilized surface.The signal amplification antibody was applied to amplify the response signal.After each measurement,the surface was regenerated with 0.1mol/L H3PO4.The assay was rapid,requiring only 30 min for antibody immobilization and 20 min for each subsequent process of immune binding,antibody amplification and regeneration.The antibody immobilized surface had good response to human IgG in the range of 0.12-60 nmol/L with a detection limit of 60 pmol/L.The same antibody immobilized surface could be used for more than 110 cycles of binding,amplification and regeneration.The results demonstrate that the sensitivity,specificity and reproducibility of amplified immunoassay using real-time BIA technology are satisfactory.
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| 关 键 词： | 人 免疫球蛋白 IgG 免疫分析 生物分子 相互作用 BIA |
Amplified Immunoassay of Human IgG Using Real-time Biomolecular Interaction Analysis (BIA) Technology |
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| PEI,Ren Jun CUI,Xiao Qiang YANG,Xiu Rong WANG,Er Kang Laboratory of Electroanalytical Chemistry and National Analytical Research Center of Electrochemistry and Spectroscopy,Changchun Institute of Applied Chemistry,Chinese Academy of Sc. Amplified Immunoassay of Human IgG Using Real-time Biomolecular Interaction Analysis (BIA) Technology[J]. Chinese Journal of Chemistry, 2002, 20(5): 441-446. DOI: 10.1002/cjoc.20020200507 |
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| Authors: | PEI Ren Jun CUI Xiao Qiang YANG Xiu Rong WANG Er Kang Laboratory of Electroanalytical Chemistry National Analytical Research Center of Electrochemistry Spectroscopy Changchun Institute of Applied Chemistry Chinese Academy of Sc |
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| Abstract: | An automated biomolecular interaction analysis instrument (BIAcore) based on surface plasmon resonance (SPR) has been used to determine human immunoglobulin G (IgG) in real time. Polyclonal anti human IgG antibody was covalently immobilized to a carboxymethyldextran modified gold film surface. The samples of human IgG prepared in HBS buffer were poured over the immobilized surface. The signal amplification antibody was applied to amplify the response signal. After each measurement, the surface was regenerated with 0.1 mol/L H 3PO 4. The assay was rapid, requiring only 30 min for antibody immobilization and 20 min for each subsequent process of immune binding, antibody amplification and regeneration. The antibody immobilized surface had good response to human IgG in the range of 0.12 -60 nmol/L with a detection limit of 60 pmol/L. The same antibody immobilized surface could be used for more than 110 cycles of binding, amplification and regeneration. The results demonstrate that the sensitivity, specificity and reproducibility of amplified immunoassay using real time BIA technology are satisfactory. |
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| Keywords: | amplified immunoassay biomolecular interaction analysis surface plasmon resonance immunoglobulin G |
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