| 毛细管电泳电致化学发光法测定牛奶样中的土霉素残留量 |
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| 引用本文： | 杨伟群,李玉杰,周敏. 毛细管电泳电致化学发光法测定牛奶样中的土霉素残留量[J]. 分析试验室, 2010, 29(11) |
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| 作者姓名： | 杨伟群 李玉杰 周敏 |
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| 作者单位： | 1. 台州职业技术学院生物与化学工程系,台州,318000
2. 西北师范大学化学化工学院,兰州,730070 |
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| 基金项目： | 浙江省教育厅2008年度科研项目(Y200806055)资助 |
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| 摘 要： | 以铕离子(Ⅲ)掺杂类普鲁士蓝(Eu-PB)化学修饰铂电极为工作电极,基于铜(Ⅱ)-土霉素配合物对三联吡啶钌(Ⅱ)电致化学发光强度的增敏作用,建立了用毛细管电泳电致化学发光法测定土霉素的新方法。实验对毛细管电泳分离条件和电化学发光检测条件进行了优化。在最佳实验条件下,电致化学发光峰面积与铜(Ⅱ)-土霉素配合物的浓度在0.138～46.1μg/mL范围内呈良好的线性关系,检出限为57.0ng/mL(3σ)。本法用于牛奶样中土霉素残留量的测定,加标回收率为95.5%(n=5)。
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| 关 键 词： | 毛细管电泳 电致化学发光 化学修饰电极 土霉素 铜(II) |
Determination of oxytetracycline residues in milk by capillary electrophoresis with electrochemiluminescence detection |
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| YANG Wei-qun,LI Yu-jie,ZHOU Min. Determination of oxytetracycline residues in milk by capillary electrophoresis with electrochemiluminescence detection[J]. Chinese Journal of Analysis Laboratory, 2010, 29(11) |
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| Authors: | YANG Wei-qun LI Yu-jie ZHOU Min |
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| Affiliation: | YANG Wei-qun 1,LI Yu-jie2and ZHOU Min2 (1.Department of Biological & Chemical Engineering,Taizhou Vocational & Technical College,Taizhou 318000,2.Chemistry & Chemical Engineering College,Northwest Normal University,Lanzhou 730070) |
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| Abstract: | Based on the enhancement of a copper (II)-oxytetracycline complex on Ru(bpy)2+3-based electrochemiluminescence (ECL) intensity,a novel ECL assay coupled with capillary electrophoresis (CE) has been established for the determination of oxytetracycline (OTC) with an Eu-PB modified platinum electrode as the working electrode.Parameters related to the separation and detection were discussed and optimized.Under the optimum conditions,ECL peak area was in proportion to concentration of Cu(II)-OTC complex in the r... |
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| Keywords: | Capillary electrophoresis Electrochemiluminescence Chemically modified electrode Oxytetracycline Copper (II) |
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