蛋白质在表面活性剂与高分子共组双水相体系中 的分配

高分子和正负离子表面活性剂混合物可形成一种新型双水相体系。研究蛋白质在溴化十二烷基三乙铵/十二烷基硫酸钠与聚氧乙烯(EO)-聚氧丙烯(PO)嵌段共聚物(EO~2~0PO~8~0)共组双水相体系中的分配。通过在高分子接上亲和配基，研究蛋白质在带有亲和配基高分子的双水相体系中的分配。将表面活性剂富集相稀释或加热高分子富集相，又可形成新的双水相体系，由此可进行蛋白质的多步分配。在蛋白质的分配完成之后，通过将表面活性剂富集相进一步稀释或将高分子富集相加热至高分子浊点以上可将表面活性剂和高分子与目标蛋白质分离。正负离子表面活性剂和高分子还可以循环使用。

Protein partitioning in aqueous two-phase systems formed by mixtures of polymers and cationic-anionic surfactants

A novel aqueous two - phase system (ATPS) can be formed by mixing a polymer and a cationic -anionic surfactant. In this work we investigated the protein partitioning in ATPS formed by mixing dodecyltriethylarnmonium bromide/sodium dodecylsulfate and ethylene oxide ( EO) - propylene oxide ( PO) random copolymers (EO20PO80) . Affinity ligands were attached to polymers and affinity partitioning of proteins was investigated. A multi - step partitioning procedure was developed by diluting the surfactant - enriched phase or by raising the temperature of polymer - enriched phase. After partitioning was completed, the surfactants could be removed when the phases were further diluted, and EO - PO copolymers could be separated by raising the temperature above the cloud point of copolymer. The recycling of cationic - anionic surfactants and EO - PO copolymers is discussed.