Wine Aroma Improvement Using a β-Glucosidase Preparation from Aureobasidium pullulans |
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Authors: | Milla A Baffi Thaise Tobal João Henrique Ghilardi Lago Maurício Boscolo Eleni Gomes Roberto Da-Silva |
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Institution: | 1. Agricultural Sciences Institute (ICIAG), Uberlandia Federal University (UFU), 38405-303, Uberlandia, MG, Brazil 2. Laboratory of Biochemistry and Applied Microbiology, S?o Paulo State University (UNESP), 15054-000, S?o José do Rio Preto, SP, Brazil 3. S?o Paulo Federal University (UNIFESP), 09972-270, Diadema, SP, Brazil
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Abstract: | Microbial β-glucosidases have been used for the enhancement of wine aroma. Nevertheless, few enzymes are active in the conditions of winemaking. In this work, the production of a β-glucosidase by an Aureobasidium pullulans strain (Ap-β-gl) isolated from grape ecosystems was evaluated. The maximum enzymatic synthesis using submerged fermentation was after 96 h of growth in complex media containing 20 g/L of cellobiose as the sole carbon source. The crude enzyme (Ap-β-gl) showed optimal pH at 5.5 and two peaks of optimum temperature (at 45 and 70 °C). It showed a wide range of pH stability, stability at low temperatures, and tolerance to ethanol, showing suitable characteristics for winemaking conditions. The hydrolysis of glycosidic terpenes by Ap-β-gl was studied, and its ability to efficiently release free terpenols was demonstrated by gas chromatography/mass spectrometry. The enzymatic treatment notably increased the amount of monoterpenes, showing good prospects for its potential application for the development of aroma in wines. |
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