Automated data reduction for hydrogen/deuterium exchange experiments,enabled by high-resolution fourier transform ion cyclotron resonance mass spectrometry |
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Authors: | Sasa Kazazic Hui-Min Zhang Tanner M Schaub Mark R Emmett Christopher L Hendrickson Gregory T Blakney Alan G Marshall |
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Institution: | 1.Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory,Florida State University,Tallahassee,USA;2.Molecular Biophysics Program,Florida State University,Tallahassee,USA;3.Department of Chemistry and Biochemistry,Florida State University,Tallahassee,USA |
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Abstract: | Mass analysis of proteolytic fragment peptides following hydrogen/deuterium exchange offers a general measure of solvent accessibility/hydrogen
bonding (and thus conformation) of solution-phase proteins and their complexes. The primary problem in such mass analyses
is reliable and rapid assignment of mass spectral peaks to the correct charge state and degree of deuteration of each fragment
peptide, in the presence of substantial overlap between isotopic distributions of target peptides, autolysis products, and
other interferant species. Here, we show that at sufficiently high mass resolving power (m/Δm50% ≥ 100,000), it becomes possible to resolve enough of those overlaps so that automated data reduction becomes possible, based
on the actual elemental composition of each peptide without the need to deconvolve isotopic distributions. We demonstrate
automated, rapid, reliable assignment of peptide masses from H/D exchange experiments, based on electrospray ionization FT-ICR
mass spectra from H/D exchange of solution-phase myoglobin. Combined with previously demonstrated automated data acquisition
for such experiments, the present data reduction algorithm enhances automation (and thus expands generality and applicability)
for high-resolution mass spectrometry-based analysis of H/D exchange of solution-phase proteins. |
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