Evaluation of DNA damage and cytotoxicity of polyurethane-based nano- and microparticles as promising biomaterials for drug delivery systems |
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Authors: | Thiago?Caon Betina?Giehl?Zanetti-Ramos Elenara?Lemos-Senna Eric?Cloutet Henri?Cramail Redouane?Borsali Valdir?Soldi Cláudia?Maria?Oliveira?Sim?es |
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Institution: | 1.Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas,Universidade Federal de Santa Catarina,Florianópolis,Brazil;2.Laboratório de Estudos em Materiais Poliméricos, Departamento de Química,Universidade Federal de Santa Catarina,Florianópolis,Brazil;3.Laboratório de Farmacotécnica, Departamento de Ciências Farmacêuticas,Universidade Federal de Santa Catarina,Florianópolis,Brazil;4.Laboratoire de Chimie des Polymères Organiques (LCPO),CNRS/ENSCPB-Université Bordeaux 1 (UMR5629),Pessac Cedex,France |
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Abstract: | The in vitro cytotoxicity and DNA damage evaluation of biodegradable polyurethane-based micro- and nanoparticles were carried
out on animal fibroblasts. For cytotoxicity measurement and primary DNA damage evaluation, MTT and Comet assays were used,
respectively. Different formulations were tested to evaluate the influence of chemical composition and physicochemical characteristics
of particles on cell toxicity. No inhibition of cells growth surrounding the polyurethane particles was observed. On the other
hand, a decrease of cell viability was verified when the anionic surfactant sodium dodecyl sulfate (SDS) was used as droplets
stabilizer of monomeric phase. Polyurethane nanoparticles stabilized with Tween 80 and Pluronic F68 caused minor cytotoxic
effects. These results indicated that the surface charge plays an important role on cytotoxicity. Particles synthesized from
MDI displayed a higher cytotoxicity than those synthesized from IPDI. Size and physicochemical properties of the particles
may explain the higher degree of DNA damage produced by two tested formulations. In this way, a rational choice of particles’
constituents based on their cytotoxicity and genotoxicity could be very useful for conceiving biomaterials to be used as drug
delivering systems. |
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