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Rapid and sensitive detection of bacteria via platinum-labeled antibodies and on-particle ionization and enrichment prior to MALDI-TOF mass spectrometry |
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| Authors: | Faheem Ahmad Hui-Fen Wu |
| Institution: | 1. Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan 5. Department of Biological Sciences, Faculty of Agriculture, Science and Technology, North-West University, Mafikeng Campus, Private Bag X2046, Mmabatho, 2735, South Africa 2. Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan 3. Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan 4. School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, 807, Taiwan |
| Abstract: | We introduce a rapid and sensitive approach to study the interactions of an affinity probe with the bacterial wall. Immunoglobulin was immobilized on platinum nanoparticles, and the resulting probe nanoparticles bind to bacterial walls as confirmed by transmission electron microscopy. A MALDI-MS assay was developed that can detect ~105 cfu mL?1 of S. marcescens and E. coli. This approach enables simple, rapid and straightforward detection of bacterial proteins, with high resolution and sensitivity, and without the requirement for tedious washing/separation steps. Antobody IgG treated Pt NPs are successfully implemented to bind the cell surfaces of target bacteria. The current bio-analytical technique allows simple, rapid and straightforward identification of bacteria. The obtained results proved that IgG modified platinum nanoparticle strategy was also capable to enhance the protein peaks with high signal intensity and resolution. |
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