Optimization of a multisolvent composition in RPHPLC: Generalization to non- ideal peaks |
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Authors: | S Heinisch J L Rocca |
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Institution: | (1) Laboratoire des Sciences Analytiques (UA CNRS 435), Université Lyon I, 43 BLVD du 11 Novembre 1918, 69622 Villeurbanne, France |
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Abstract: | Summary When samples contain solutes at very different concentrations, the use of the classical resolution function to express the
quality of the separation does not provide a true measure of peak overlap and is therefore unsuitable for optimization. Apart
from this case, the validity of the optimum is highly dependent on the accuracy of the predicted retention surfaces and it
is essential to restrict the parameter space in which optimum performance is sought. As a result, most interpretive optimization
procedures become unreliable for optimizing the composition of multisolvent systems in RPHPLC, particularly for non-ideal
peaks. These difficulties can be overcome by the method proposed here: the ability, by means of a criterion, called CRIT,
to select a restricted range of composition in order to enhance the accuracy of the response surface is developed for non-ideal
peaks. The best composition within this selected range is then determined by using a modified resolution function. The performance
of this procedure is discussed with some illustrative examples. |
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Keywords: | Reversed phase HPLC (RPHPLC) Optimization Multisolvent mobile phases Non ideal peaks |
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