Long-lifetime metal-ligand complexes as luminescent probes for DNA |
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| Authors: | Henryk Malak Ignacy Gryczynski Joseph R Lakowicz Gerald J Meyers Felix N Castellano |
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| Institution: | (1) Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 West Lombard Street, 21201 Baltimore, Maryland;(2) Department of Chemistry, Johns Hopkins University, 3400 Charles Street, 21218 Baltimore, Maryland |
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| Abstract: | We examined the intensity and anisotropy decays of DNA labeled with two ruthenium metalligand complexes, Ru(bpy)2(dppz)]2+ and Ru(phe)2(dppz)]2+. Both complexes display high emission anisotropies in the absence of rotational diffusion, making them suitable probes for
rotational motions. When bound to DNA, these complexes display decay times as long as 294 ns, providing long-lived probes
of DNA dynamics. The decay times of both complexes were rather insensitive to dissolved oxygen. We examined anisotropy decays
of these complexes bound to B-form DNA. The anisotropy decays revealed correlation times near 10, 50, and several hundred
nanoseconds, suggesting that these probes are sensitive to a wide range of DNA motions. The use of metalligand complexes should
allow resolution of both the torsional and bending motions of DNA, the latter of which has been mostly inaccessible using
shorter-lived fluorescent probes bound to DNA.
Dedicated to Professor Robert F. Steiner upon his retirement |
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| Keywords: | Metal-ligand complexes luminescent probes DNA ruthenium (II) complexes DNA intercalator |
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