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| 傅里叶变换红外光谱法研究PLGA微球中葡激酶突变体(K35R)的二级结构 | |
| 引用本文： | 贺进田,王改珍,宋后燕.傅里叶变换红外光谱法研究PLGA微球中葡激酶突变体(K35R)的二级结构[J].光谱学与光谱分析,2006,26(7):1209-1212. |
| 作者姓名： | 贺进田 王改珍 宋后燕 |
| 作者单位： | 1. 河北师范大学生命科学学院，河北 石家庄 050016 2. 复旦大学分子医学教育部重点实验室，上海 200032 3. 河北科技大学环境科学与工程学院，河北 石家庄 050018 |
| 基金项目： | 高比容电子铝箔的研究开发与应用项目 |
| 摘 要： | 用复乳溶剂挥发法制备了葡激酶突变体(K35R，DGR)的PLGA微球，并用傅里叶变换红外光谱法(FTIR)定量研究了PLGA微球内DGR的二级结构。 将可增强分辨率的傅里叶去卷积技术与高斯曲线拟合技术共同用于对微球内DGR酰胺Ⅰ带的定量分析，发现DGR酰胺Ⅰ带共包含9个红外吸收峰，并对各组份进行了归属。 此外，还对微球内DGR结构稳定性相关的1 623和1 650 cm-1吸收峰进行了讨论。 |
| 关 键 词： | 葡激酶 微球 傅里叶变换红外光谱 |
| 文章编号： | 1000-0593（2006）07-1209-04 |
| 收稿时间： | 2005-05-23 |
| 修稿时间： | 2005-08-24 |
FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres |
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| HE Jin-tian,WANG Gai-zhen,SONG Hou-yan.FTIR Characterization of the Secondary Structure of a Staphylokinase Variant (K35R) Encapsulated within PLGA Microspheres[J].Spectroscopy and Spectral Analysis,2006,26(7):1209-1212. | |
| Authors: | HE Jin-tian WANG Gai-zhen SONG Hou-yan |
| Institution: | 1. College of Life Science, Hebei Normal University, Shijiazhuang 050016, China2. Key Laboratory of Molecular Medicine, Ministry of Education, Fudan University, Shanghai 200032, China3. College Laboratory Environmental Science and Engineering, Hebei University of Science and Technology Shijiazhuang 050018, China |
| Abstract: | The secondary structure of a staphylokinase variant (K35R, DGR) encapsulated in poly (lactic-co-glycolic acid) (PLGA) microspheres was quantitatively examined by Fourier transform infrared (FTIR) spectroscopy. Resolution enhancement technique and Fourier deconvolution were combined with band with curve-fitting procedures to quantitate the spectral information from the amide I bands. Nine component bands were found under the broad, nearly featureless amide I bands and assigned to alpha-helix, beta-sheet, turn and irregular (random) structures. The changes of bands at 1 651 and 1 623 cm(-1) after encapsulation were discussed. |
| Keywords: | Staphylokinase Microsphere FTIR |
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