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Intercalation-induced changes in DNA supercoiling observed in real-time by atomic force microscopy |
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| Authors: | L. H. Pope M. C. Davies C. A. Laughton C. J. Roberts S. J. B. Tendler P. M. Williams |
| Affiliation: | a Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham NG7 2RD, UK b Cancer Research Laboratories, School of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham NG7 2RD, UK |
| Abstract: | Atomic force microscopy (AFM) has been employed to observe in real-time and in an aqueous environment the process of ethidium bromide induced supercoiling in individual DNA plasmid molecules. Image data reveal both the onset and the progressive presence of plectonemic DNA supercoiling. In addition, significant molecular motion of the surface adsorbed DNA is observed. These data illustrate the potential of AFM in the time-resolved study of biomolecular processes, and hence, provide new insights into biomolecular structure and function. |
| Keywords: | Atomic force microscopy DNA Supercoiling Ethidium bromide |
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