Radioimmunoassay of phenytoin |
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Authors: | G Samuel N Sivaprasad R S Mani |
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Institution: | (1) Isotope Division, Bhabha Atomic Research Centre, Trombay, 400085 Bombay, (India);(2) Radiopharmaceuticals Operation, BRIT, New Bombay, (India);(3) Chemistry and Industrial Application Section, International Atomic Energy Agency, Wagramerstrasse 5, P.O.Box 200, A-1400 Vienna, (Austria) |
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Abstract: | We describe a radioimmunoassay procedure for the measurement of phenytoin (5,5-diphenylhydantoin) in serum samples. Antiserum to phenytoin has been produced against phenytoin-valeric acid-bovine serum albumin conjugate.125I labelled phenytoin-acetic acid-tyrosine methyl ester has been used as a tracer. The assay covers a range of 10–500 ng/cm3 and has a sensitivity of 0.25 ng. The assay is validated by specificity tests, precision profile and recovery tests. |
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