Parisaponin I 和Parisvietnaside A 的NMR数据分析

从滇重楼中分离得到2个甾体皂苷，利用¹H NMR、¹³C NMR、¹H-¹H COSY、HSQC、 HMBC、1D和2D TOCSY等多种核磁共振方法鉴定其结构分别为(25R)-26-O-β-D-吡喃葡萄糖基-3β, 22α, 26-三羟基-呋甾-5-烯- 3-O-α-L -吡喃鼠李糖基-(1→2)α-L-呋喃阿拉伯糖基(1→4)]-β-D-吡喃葡萄糖苷（1, Parisaponin I）和(25R)-3β, 5α, 6β-三羟基-△7-螺甾烯-3-O-β-D-吡喃葡萄糖基-(1→3)α-L-吡喃鼠李糖基-(1→2)]-β-D-吡喃葡萄糖苷（2， parisvietnaside A）. 对化合物1和2的¹H NMR和¹³C NMR信号分别进行了归属和详细分析，并纠正了文献中的核磁数据归属错误. 化合物2为首次从滇重楼中分离得到. 

An NMR Study on Parisaponin I and Parisvietnaside A Isolated from Paris polyphylla Smith var. yunnanensis

Two steroidal saponins were isolated from the rhizome of Paris polyphyllaSmith var. yunnanensis. Their structures were elucidated as (25R)-26-O-β-D-glucopyranosyl-3β, 22α, 26-trihydroxy-furost-5-ene-3-O-α-L- rhamnopyranosyl-(1→2)-α-L-arabinofuranosyl-(1→4)]-β-D-glucopyranoside (1, parisaponin I) and (25R)-3β, 5α, 6β-trihydroxy-7(8)-ene-spirostanol-3-O-β- D-glucopyranosyl-(1→3)-α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (2, parisvietnaside A) by MS, 1D and 2D NMR spectroscopy. The ¹H and ¹³C chemical shifts of the compounds were assigned, and the incorrect assignments in previous literatures were corrected. Compound 2 was isolated from the plant for the first time.