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Reversed-phase high-performance liquid chromatographic separation of epimeric alkaloid N-oxides from Thalictrum simplex L. |
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| Authors: | Maria P Velcheva Christa Werner Manfred Hesse |
| Institution: | a Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria b Organisch-chemisches Institut, Universität Zürich, Winterthurerstrasse 190, 8057, Zürich, Switzerland |
| Abstract: | A simple and precise method was developed for the analytical and preparative reversed-phase HPLC separation of a mixture of epimeric pavine N-oxides containing 49.1% of (?)-thalimonine N-oxide A and 50.1% of (?)-thalimonine N-oxide B isolated from Thalictrum simplex L. (Ranunculaceae). A reversed-phase system with Nucleosil C18 analytical and preparative columns and ethanol-1.5% aqueous orthophosphoric acid (15:85) as the mobile phase was used. The epimeric pavine N-oxides were completely separated within 50 min. |
| Keywords: | Thalictrum simplex L Alkaloid N-oxides Thalimonine N-oxides Pavine N-oxides |
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