| 岱衢族大黄鱼不同组织的同工酶谱 |
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| 引用本文： | 管丹冬,李明云,叶帅东.岱衢族大黄鱼不同组织的同工酶谱[J].宁波大学学报(理工版),2008,21(1):34-38. |
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| 作者姓名： | 管丹冬 李明云 叶帅东 |
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| 作者单位： | 宁波大学,生命科学与生物工程学院,浙江,宁波,315211 |
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| 基金项目： | 国家高技术研究发展计划 3项目
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浙江省重点项目
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浙江省宁波市重大招标项目 |
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| 摘 要： | 于2006年11月,在象山港采集30尾网养岱衢族大黄鱼,采用聚丙烯酰胺凝胶垂直电泳方法,研究分析了岱衢族大黄鱼眼睛、肌肉、肝脏、肾脏、脾脏、心脏、胸鳍、鳃和脑9种组织中16种同工酶（LDH、ADH、MDH、ME、SOD、POD、EST、GAD、ALP、ATP、FDH、SCD、IDH、GDH、ACP、GcDH）的表达情况,发现除IDH、GDH、ACP、GcDH 4种酶只显示微弱且不稳定的区带外,其他酶类在各组织中都显示出清晰稳定的酶谱,且这些酶在表型、分布和活性上均表现出高度的组织特异性,与各组织的生理功能相一致.
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| 关 键 词： | 岱衢族大黄鱼 同工酶 组织特异性表达 |
Isozyme Patters in Various Tissues of Dai-ju Stock Pseudosciaena crocea |
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| GUAN Dan-dong,LI Ming-yun,YE Shuai-dong.Isozyme Patters in Various Tissues of Dai-ju Stock Pseudosciaena crocea[J].Journal of Ningbo University(Natural Science and Engineering Edition),2008,21(1):34-38. |
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| Authors: | GUAN Dan-dong LI Ming-yun YE Shuai-dong |
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| Institution: | ( Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211., China ) |
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| Abstract: | In November, 2006, 30 Dai-ju Stock Pseudosciaena crocea samples were collected in Xiangshan bay, Zhejiang Province. Vertical polyacrylamide gel electrophoresis was used to investigate the expression of sixteen isozymes （LDH, ADH, MDH, ME, SOD, POD, EST, GAD, ALP, ATP, FDH, SCD, IDH, GDH, ACP, GcDH） in 9 tissues （eye, muscle, liver, kidney, spleen, heart, pectroral fin, gill and brain） of the samples. The result indicate that, except 4 isozymes showing weak and unsteady bands, the remaining isozymes reveal clear and steady bands in all the tissues of various kinds. |
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| Keywords: | Dai-ju stock Pseudosciaena crocea isozyme tissue specific expression |
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