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大肠杆菌分泌型穿梭质粒P^#GTE5DNA的酶切图谱
引用本文:决肯·阿努瓦什.大肠杆菌分泌型穿梭质粒P^#GTE5DNA的酶切图谱[J].新疆大学学报(理工版),1989,6(2):98-101.
作者姓名:决肯·阿努瓦什
作者单位:新疆八一农学院生物基础部遗传教研室
摘    要:本文采用碱性裂解法获得产率较高的分泌型穿梭质粒P~# GTE_5 DNADNA,并作限制性内切酶的单、双酶完全消化,通过对DNA电泳条带的分析,初步得出该质粒的限制性酶切图谱.

关 键 词:大肠杆菌  穿梭质粒    切图谱  DNA

Enzyme Picture of Secrete Shuttle Plasmid P~# GTE_6 DNA of E.coli
Joken Anuax.Enzyme Picture of Secrete Shuttle Plasmid P~# GTE_6 DNA of E.coli[J].Journal of Xinjiang University(Science & Engineering),1989,6(2):98-101.
Authors:Joken Anuax
Abstract:Much secrete and splitting methods shuttle plasmid P~#GTE_5DNA was made byusing lase enzyme.The research to complete digestion of restriction enzymes forsingle-enzyme and bienzyme was also done.The enzyme fragments were seperatedfrom the digested plasmid P~#GTE_5DNA with electro phoresis of 1.5% agarasegel.By the analysis of DNA fragments,the picture of restriction enzyme of the plasmidwas got.
Keywords:shuttle plasmid P~#GTE_5  enzyme  picture  secrete
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