Protein separation using preparative-scale dynamic field gradient focusing |
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Authors: | Tracy Noah I Ivory Cornelius F |
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Affiliation: | School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, USA. |
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Abstract: | Dynamic field gradient focusing uses an electric field gradient to separate and concentrate proteins in native buffers. A prototype preparative-scale dynamic field gradient focusing apparatus reproducibly separated hemoglobin and bovine serum albumin with a mean resolution of 2.64+/-0.503. Run-to-run variations in the hemoglobin's focal point and peak width appeared to be related to fluctuations in the shape of the electric field, rather than the 5% accuracy of the pump that provided the counter-flow in the separation annulus. The variation in the electric field gradient was probably due to the formation and expansion of an ion-depleted region at the top of the separation annulus. |
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Keywords: | Dynamic field gradient focusing Electric field gradient focusing Equilibrium‐gradient method Preparative electrophoresis |
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