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A new thermostable endoglucanase,Acidothermus cellulolyticus E1 |
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| Authors: | Baker John O Adney William S Nleves Rafael A Thomas Steven R Wilson David B Himmel Michael E |
| Institution: | 1.Alternative Fuels Division, National Renewable Energy Laboratory, 1617 Cole Blvd., 80401, Golden, CO ;2.Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY ; |
| Abstract: | A new thermostable endoglucanase,Acidothermus cellulolyticus E1, and another bacterial endoglucanase, E5 fromThermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case ofA. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time. |
| Keywords: | Index Entries" target="_blank">Index Entries Acidothermus cellulolyticus E1 Thermomonospora fusca E5 Trichoderma reesei CBH I synergism endoglucanase |
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