How polyamine synthesis inhibitors and cinnamic acid affect tropane alkaloid production |
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Authors: | Patricia L Marconi María A Alvarez Sandra I Pitta-Alvarez |
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Institution: | (1) CONICET, Fundación Pablo Cassará, Centro de Ciencia y Tecnología Dr César Milstein, Ciudad Autónome de Buenos Aires, 2452 (1440FFX) Saladillo, Argentina |
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Abstract: | Hairy roots of Brugmansia candida produce the tropane alkaloids scopolamine and hyoscyamine. In an attempt to divert the carbon flux from competing pathways
and thus enhance productivity, the polyamine biosynthesis inhibitors cyclohexylamine (CHA) and methylglyoxal-bis-guanylhy,
drazone (MGBG) and the phenylalamine-ammonia-lyase inhibitor cinnamic acid were used. CHA decreased the specific productivity
of both alkaloids but increased significantly the release of scopolamine (approx 500%) when it was added in the mid-exponential
phase. However, when CHA was added for only 48 h during the exponential phase, the specific productivity of both alkaloids
increased (approx 200%), favoring scopolamine. Treatment with MGBG was detrimental to growth but promoted release into the
medium of both alkaloids. However, when it was added for 48 h during the exponential phase, MGBG increased the specific productivity
(approx 200%) and release (250–1800%) of both alkaloids. Cinnamic acid alone also favored release but not specific productivity.
When a combination of CHA or MGBG with cinnamic acid was used, the results obtained were approximately the same as with each
polyamine biosynthesis inhibitor alone, although to a lesser extent. Regarding root morphology, CHA inhibited growth of primary
roots and ramification. However, it had a positive effect on elongation of lateral roots. |
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Keywords: | Brugmansia candida scopolamine hyoscyamine cyclohexylamine methylglyoxal-bis-guanylhydrazone trans-cinnamic acid tropane alkaloids |
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