In situ analysis of trace elements in metalloproteins of human liver by synchrotron radiation X-ray fluorescence |
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| Authors: | Chunying Chen Peiqun Zhang Zhifang Chai Guangcheng Li and Yuying Huang |
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| Institution: | (1) Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, 100080 Beijing, China;(2) Synchrotron Radiation Laboratory, Institute of High Energy Physics, Chinese Academy of Sciences, 100080 Beijing, China |
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| Abstract: | A method using synchrotron radiation X-ray fluorescence analysis toin situ determine trace elements during protein electrophoretically separating process was established. The distribution of elements
in protein bands for human liver cytosolic sample separated by SDS-PAGE was analyzed along polyacrylamide gel. The results
showed that the protein fraction of peak III in cytosol was mainly composed of metal ion Zn-associated proteins, being in
agreement with that given by atomic absorption spectrometry. Thus, it demonstrated the feasibility of this novel technique
forin situ analysis of trace elements in protein bands. |
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| Keywords: | synchrotron radiation X-ray fluorescence analysis in situ analysis metalloprotein electrophoreticseparation |
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