High-performance liquid chromatography of sugars on copper (II) modified silica gel |
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| Authors: | J. L. Leonard F. Guyon P. Fabiani |
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| Affiliation: | (1) Laboratoire de Biochimie, Hôpital Hôtel-Dieu, Place du Parvis Notre-Dame, 75181 Paris Cedex 04, France |
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| Abstract: | Summary A sensitive method of sugar analysis by HPLC is described in which a copper (II) modified silica gel as stationary phase is used. Detection is based on UV absorption of a complex formed between the sugar, copper, and ammonia. |
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| Keywords: | Column liquid chromatography Sugars Copper (II) complex UV detection |
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