HPLC of aminoacids on silica coated with ammonium tungstophosphate |
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Authors: | L Lepri P G Desideri L Merlini L Checchini |
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Institution: | (1) Istituto di Chimica Analitica, Università di Firenze, Via G. Capponi, 9, 50121 Florence, Italy |
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Abstract: | Summary The chromatographic behaviour of aminoacids on columns of silica coated with different percentages of ammonium tungstophosphate at several buffer phosphate concentrations is reported. The stationary phase shows a high selectivity towards aminobutyric acids and methyl derivatives of tryptophan due to the distance between the carboxylic group and the –NH
3
+
group involved in the cation-exchange process and/or to the steric hindrance by a methyl substituent. A relationship between HPLC and HPTLC data has been verified and applied to the prediction of the separation of six aminoacids. |
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Keywords: | Column liquid chromatography Thin layer chromatography Ammonium tungstophosphate Aminoacids |
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