毫微秒荧光寿命的解卷积处理

本文介绍毫微秒荧光寿命的解卷积原理及实验系统与实验方法,讨论了数据精确性判别并举出若干例子。此外,我们结合实际需要还对数据处理软件进行若干扩充,扩大了数据处理功能。

DECONVOLUTION TREATMENT FOR NANOSECOND FLUORESCENCE LIFETIME

In recent years, the measurement of fluorescence lifetime, decay function and time resolved spectra have made an increasing contribution to the understanding of rapid processes in physical, Chemical and biological sciences. These measurements carried out on microsecond to picosecond time scale, it requires using the single photon counting technique. The decay functions are analysed using numerical convolution and devolution. The experimental data are fitted to preselected kinetic models using rigorous statistical methods. Based on the model SP-70 nanosecond fluorescence spectrometer, this paper disscusses data analysis of fluorescence lifetime, convolution mathematical expression, statistical tests to judge the quality of the results and deconvoluti-on treatment of nanosecond fluorescence lifetime.