Covalent attaching protein to graphene oxide via diimide-activated amidation |
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| Affiliation: | 1. College of Sciences, Shanghai University, Shanghai 200444, PR China;2. Department of Surgery, Shanghai Xinhua Hospital, Shanghai Jiaotong University, Shanghai 200092, PR China;3. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China;1. Division of Life Science and State Key Laboratory of Molecular Neuroscience, Center of Systems Biology and Human Health, The Hong Kong University of Science and Technology, Clearwater Bay, Kowloon, Hong Kong, China;2. Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China;3. Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, 200240, China;4. Department of Developmental Biology, School of Basic Medical Sciences, South China University of Technology, Guangzhou, 510630, China;5. Division of Life Science, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China |
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| Abstract: | In this paper, graphene oxide nanosheets (GOS) are functionalized by bovine serum albumin (BSA) via diimide-activated amidation under ambient conditions. The obtained GOS–BSA conjugate is highly water-soluble. Results of atomic force microscopy (AFM), Raman spectra and Fourier transform infrared spectroscopy analysis confirm that GOS–BSA conjugate contains both GOS and BSA protein. AFM image shows that GOS are fully exfoliated. Results of cyclic volatammograms show that the protein in the GOS–BSA conjugate retains its bioactivity. The present method may also provide a way to synthesize graphene-based composites with other biomolecules. |
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