| 一种示踪干细胞的磁共振-荧光双模成像探针 |
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| 引用本文： | 赵敏敏,张艳辉,张朋利,谭波,蒋海珍,张海禄,邓宗武. 一种示踪干细胞的磁共振-荧光双模成像探针[J]. 波谱学杂志, 2019, 36(2): 127-137. DOI: 10.11938/cjmr20182659 |
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| 作者姓名： | 赵敏敏 张艳辉 张朋利 谭波 蒋海珍 张海禄 邓宗武 |
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| 作者单位： | 上海大学 理学院,上海 200444;中国科学院 苏州纳米技术与纳米仿生研究所,江苏 苏州 215123;中国科学院 苏州纳米技术与纳米仿生研究所,江苏 苏州,215123;上海大学 理学院,上海,200444 |
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| 基金项目： | the National Natural Science Foundation of China (31371010, 21673281); Funds from Youth Innovation Promotion Association of the Chinese Academy of Sciences (2012242); Key Research Project from the Ministry of Science and Technology of China (2017YFA0104300). |
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| 摘 要： | 本文设计并合成了Gd基磁共振-荧光双模成像探针——Gd-DOTA-PEG-GA，通过电穿孔的方式标记人源间充质干细胞（hMSCs）.电穿孔标记诱导细胞将探针组装成团簇状纳米粒子进入细胞质，显著延长其与细胞结合的时间，并呈现出明显的T2信号减弱效应，且信号减弱效应可以持续7天以上.在水溶液中，该探针的发射带集中在498 nm，并且荧光强度在一周内无明显衰减.该探针标记的细胞在荧光倒置显微镜下呈现绿色荧光.这些结果表明该探针可以作为磁共振-荧光双模成像探针用于干细胞示踪.
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| 关 键 词： | 磁共振成像 荧光成像 双模探针 人源间充质干细胞 |
| 收稿时间： | 2018-06-06 |
A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking |
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| ZHAO Min-min,ZHANG Yan-hui,ZHANG Peng-li,TAN Bo,JIANG Hai-zhen,ZHANG Hai-lu,DENG Zong-wu. A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 127-137. DOI: 10.11938/cjmr20182659 |
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| Authors: | ZHAO Min-min ZHANG Yan-hui ZHANG Peng-li TAN Bo JIANG Hai-zhen ZHANG Hai-lu DENG Zong-wu |
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| Affiliation: | 1. College of Sciences, Shanghai University, Shanghai 200444, China;2. Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China |
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| Abstract: | A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self-assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T2-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo. |
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| Keywords: | magnetic resonance imaging fluorescent imaging dual-mode probe human mesenchymal stem cell |
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