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| Bioinformatics analysis of two-component regulatory systems in Staphylococcus epidermidis | |
| 作者姓名： | QINZhiqiang ZHONGYang ZHANGJian HEYouyu WUYang JIANGJuan CHENJiemin LUOXiaomin QUDi |
| 作者单位： | [1]MinistryofEducationandHealthKeyLaboratoryforMedicalMolecularVirology,MedicalSchoolofFudanUniversity,Shanghai200032,China [2]MinistryofEducationKeyLaboratoryforBiodiversityScienceandEcologicalEngineering,SchoolofLifeSciences.FudanUniversity,Shanghai200433,China [3]DrugDiscoveryandDesignCenter,StateKeyLaboratoryofDrugResearch,ShanghaiInstituteofMateriaMedica,ChineseAcademyofSciences,Shanghai201203,China [4]BioinformaticsCenter,ShanghaiInstitutesforBiologicalStudies,ChineseAcademyofSciences,Shanghai200031,China |
| 摘 要： | Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese National Human Genome Center at Shanghai, by using bioinformatics analysis. Comparative analysis of the twocomponent regulatory systems in S. epidermidis and that of S.aureus and Bacillus sublilis shows that these systems may regulate some important biological functions, e.g. growth,biofilm formation, and expression of virulence factors in S.epidermidis. Two conserved domains, i.e. HATPase_c and REC domains, are found in all 16 pairs of two-component proteins.Homologous modelling analysis indicates that there are 4 similar HATPase_c domain structures of histidine kinases and 13 similar REC domain structures of response regulators,and there is one AMP-PNP binding pocket in the HATPase_c domain and three active aspartate residues in the REC domain. Preliminary experiment reveals that the bioinformatics analysis of the conserved domain structures in the two-component regulatory systems in S. epidermidis may provide useful information for discovery of potential drug target. |
| 关 键 词： | 生物信息学 二元调整系统 葡萄状球菌表皮菌 组氨酸激酶 反应调节器 药物目标 |
| 收稿时间： | 2004-02-18 |
| 修稿时间： | 2004-05-08 |
Bioinformatics analysis of two-component regulatory systems in Staphylococcus epidermidis |
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| QINZhiqiang ZHONGYang ZHANGJian HEYouyu WUYang JIANGJuan CHENJiemin LUOXiaomin QUDi.Bioinformatics analysis of two-component regulatory systems in Staphylococcus epidermidis[J].Chinese Science Bulletin,2004,49(12):1267-1271. | |
| Authors: | Qin Zhiqiang Zhong Yang Zhang Jian He Youyu Wu Yang Jiang Juan Chen Jiemin Luo Xiaomin Qu Di |
| Institution: | 1. Ministry of Education and Health Key Laboratory for Medical Molecular Virology, Medical School of Fudan University, 200032, Shanghai, China 2. Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University, 200433, Shanghai, China 3. Shanghai Center for Bioinformation Technology, 201203, Shanghai, China 4. Drug Discovery and Design Center, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 201203, Shanghai, China 5. Bioinformatics Center, Shanghai Institutes for Biological Studies, Chinese Academy of Sciences, 200031, Shanghai, China |
| Abstract: | Sixteen pairs of two-component regulatory systems are identified in the genome of Staphylococcus epidermidis ATCC12228 strain, which is newly sequenced by our laboratory for Medical Molecular Virology and Chinese National Human Genome Center at Shanghai, by using bio- informatics analysis. Comparative analysis of the two- component regulatory systems in S. epidermidis and that of S. aureus and Bacillus subtilis shows that these systems may regulate some important biological functions, e.g. growth, biofilm formation, and expression of virulence factors in S. epidermidis. Two conserved domains, i.e. HATPase_c and REC domains, are found in all 16 pairs of two-component proteins. Homologous modelling analysis indicates that there are 4 similar HATPase_c domain structures of histidine kinases and 13 similar REC domain structures of response regulators, and there is one AMP-PNP binding pocket in the HATPase_c domain and three active aspartate residues in the REC domain. Preliminary experiment reveals that the bioinfor- matics analysis of the conserved domain structures in the two-component regulatory systems in S. epidermidis may provide useful information for discovery of potential drug target. |
| Keywords: | Staphylococcus epidermidis two-component regulatory system histidine kinase response regulator bioinformatics drug target |
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