Effect of La on heat production by mitochondria isolated from hybrid rice |
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Authors: | Jie Dai Chuan-Ling Li Ye-Zhong Zhang Qi Xiao Ke-Lin Lei Yi Liu |
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Institution: | aCollege of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434025, China bState Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China |
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Abstract: | The effect of lanthanum on mitochondria isolated from hybrid rice Fengyou 559 (Oryza sativa L.) was investigated. Through in vivo culture, low-dose La3+ promoted, but higher dose La3+, restrained mitochondrial heat production. However, through in vitro incubation, La3+ manifested only inhibitory action on mitochondrial energy turnover, the concentration required for 50% and 100% inhibition being 50.9 and 230.2 μM (57.6 nmol/mg protein), respectively. In addition, La3+, like Ca2+, induced rice mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor cyclosporine A (CsA). The induction approached a constant limitation while mitochondrial metabolism was completely prevented by La3+, and microscopy observation showed a high disruption of inner mitochondrial membrane in this state. These results demonstrated that lanthanum influenced rice mitochondria in vivo and in vitro via different action pathways, and the latter involved the opening of rice mitochondrial permeability. |
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Keywords: | Mitochondria Metabolism La3+ Mechanism Permeability transition |
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