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Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post‐translationally Modified Peptide Ustiloxin B in Filamentous Fungi |
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| Authors: | Ying Ye Dr Atsushi Minami Yuya Igarashi Dr Myco Umemura Dr Nozomi Nagano Prof?Dr Masayuki Machida Dr Teppei Kawahara Prof?Dr Kazuo Shin‐ya Prof?Dr Katsuya Gomi Prof?Dr Hideaki Oikawa |
| Institution: | 1. Division of Chemistry, Graduate School of Science, Hokkaido University, Sapporo, Japan;2. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Sapporo, Japan;3. National Institute of Advanced Industrial Science and Technology (AIST), Koto-ku, Tokyo, Japan;4. Biomedicinal Information Research Center (BIRC), Japan Biological Informatics Consortium (JBIC), Koto-ku, Tokyo, Japan;5. Graduate School of Agricultural Science, Tohoku University, Sendai, Japan |
| Abstract: | The biosynthetic machinery of the first fungal ribosomally synthesized and post‐translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side‐chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5′‐phosphate (PLP)‐dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi. |
| Keywords: | biosynthesis heterologous expression natural products RiPPs ustiloxins |