3种不同提取方法测定百癣夏塔热片中的总黄酮

采用索氏提取、超声提取、微波萃取对百癣夏塔热片中总黄酮进行提取.3种方法均采用20倍(mL/g)甲醇;其中索氏提取时间为6h;超声提取时间为1h;微波萃取辐射时间:10min;温度:100C.以芦丁为标准品,用分光光度法测定,波长为510nm,芦丁浓度与吸光度呈良好的线性关系(r=0.9999).微波萃取、索氏提取和超...

Determination of Total Flavonoids in Baixuan Xiatare Tablets by Three Different Extraction Methods

The total flavonoids of Baixuan Xiatare Tablets were extracted by Soxhlet extraction,supersonic extraction and microwave extraction.The optimum technological conditions for the three methods were as follows：the ratio of solid-liquid（methanol） was 1∶20（g/mL）,extraction time of Soxhlet extraction was 6h,supersonic extraction was 1h and the radiation time of microwave extraction was 10min at 100℃.The determination method of total flavonoids content in Baixuan Xiatare Tablets with spectrophotometry was studied with rutin as standard substance at the wavelength of 510nm.There was a good linear relationship between concentration of rutin and absorbance（r=0.9999）.Microwave extraction had the advantages of shorter extraction time,less solvent dosage,high efficiency,energy-saving,simple operation and higher yield compared with Soxhlet extraction.Microwave extraction method is the ideal extraction,and deserves popularization and application.